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flow cytometry staining buffer  (Thermo Fisher)
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Thermo Fisher flow cytometry staining buffer
Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
Flow Cytometry Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+cytometry/pmc13068824-328-4-9?v=Thermo+Fisher
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flow cytometry staining buffer - by Bioz Stars, 2026-07
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flow cytometry  (Stratedigm Inc)
86
Stratedigm Inc flow cytometry
Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
Flow Cytometry, supplied by Stratedigm Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+cytometry/us12653896-1405-9-11?v=Stratedigm+Inc
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flow cytometry  (Protein Sciences Inc)
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Protein Sciences Inc flow cytometry
Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
Flow Cytometry, supplied by Protein Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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flow cytometry instrument  (Partec)
86
Partec flow cytometry instrument
Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
Flow Cytometry Instrument, supplied by Partec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+cytometry/pm42270693-44-12-17?v=Partec
Average 86 stars, based on 1 article reviews
flow cytometry instrument - by Bioz Stars, 2026-07
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flow cytometry 161  (Partec)
86
Partec flow cytometry 161
Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
Flow Cytometry 161, supplied by Partec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
flow cytometry 161 - by Bioz Stars, 2026-07
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flow cytometry  (Partec)
86
Partec flow cytometry
Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
Flow Cytometry, supplied by Partec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+cytometry/pm42270693-42-25-28?v=Partec
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flow cytometry - by Bioz Stars, 2026-07
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flow cytometry  (Cold Spring Harbor Laboratory Meetings)
86
Cold Spring Harbor Laboratory Meetings flow cytometry
Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
Flow Cytometry, supplied by Cold Spring Harbor Laboratory Meetings, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry data  (Dotmatics Limited)
86
Dotmatics Limited flow cytometry data
Flow <t>cytometry</t> for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.
Flow Cytometry Data, supplied by Dotmatics Limited, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry tubes  (Sarstedt)
86
Sarstedt flow cytometry tubes
Phenotypic and functional effects of a SLy1 knockout on splenic NK cells in dependence on p53. (A) Absolute NK cell count determined via flow <t>cytometry</t> in the spleen of mice of the specified genotypes. (B) Apoptotic (defined as Annexin V + ) NK cells. (C) Living (defined as 7-AAD - ) NK cells after four hours under culture conditions. (D) Expression of NKG2D, NK1.1 and NKp46 on NK cells. (E) In vitro cytotoxicity against LLC at a 25:1 effector:target ratio. Dead LLC cells were defined as 7-AAD + /Annexin V + . In all figures error bars represent standard error of the mean. ∗: p <0.05, ∗∗/##: p <0.01, ns, not significant; unpaired t-test. (F) Freshly isolated NK cells (1) attack and lyse LLC cells (2) in vitro (see arrow 3). Image was taken after four hours of incubation. Scale bar = 20 µm.
Flow Cytometry Tubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+cytometry/pmc13275445-95-7-10?v=Sarstedt
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flow cytometry tubes - by Bioz Stars, 2026-07
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Image Search Results


Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Journal: STAR Protocols

Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

doi: 10.1016/j.xpro.2026.104471

Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

Techniques: Flow Cytometry, Isolation, Staining

Phenotypic and functional effects of a SLy1 knockout on splenic NK cells in dependence on p53. (A) Absolute NK cell count determined via flow cytometry in the spleen of mice of the specified genotypes. (B) Apoptotic (defined as Annexin V + ) NK cells. (C) Living (defined as 7-AAD - ) NK cells after four hours under culture conditions. (D) Expression of NKG2D, NK1.1 and NKp46 on NK cells. (E) In vitro cytotoxicity against LLC at a 25:1 effector:target ratio. Dead LLC cells were defined as 7-AAD + /Annexin V + . In all figures error bars represent standard error of the mean. ∗: p <0.05, ∗∗/##: p <0.01, ns, not significant; unpaired t-test. (F) Freshly isolated NK cells (1) attack and lyse LLC cells (2) in vitro (see arrow 3). Image was taken after four hours of incubation. Scale bar = 20 µm.

Journal: Frontiers in Immunology

Article Title: SLy1-deficiency results in functional impaired, exhausted and senescent NK cells

doi: 10.3389/fimmu.2026.1836862

Figure Lengend Snippet: Phenotypic and functional effects of a SLy1 knockout on splenic NK cells in dependence on p53. (A) Absolute NK cell count determined via flow cytometry in the spleen of mice of the specified genotypes. (B) Apoptotic (defined as Annexin V + ) NK cells. (C) Living (defined as 7-AAD - ) NK cells after four hours under culture conditions. (D) Expression of NKG2D, NK1.1 and NKp46 on NK cells. (E) In vitro cytotoxicity against LLC at a 25:1 effector:target ratio. Dead LLC cells were defined as 7-AAD + /Annexin V + . In all figures error bars represent standard error of the mean. ∗: p <0.05, ∗∗/##: p <0.01, ns, not significant; unpaired t-test. (F) Freshly isolated NK cells (1) attack and lyse LLC cells (2) in vitro (see arrow 3). Image was taken after four hours of incubation. Scale bar = 20 µm.

Article Snippet: 10 6 single cells were transferred into flow cytometry tubes (Sarstedt) and were washed once with cold PBS.

Techniques: Functional Assay, Knock-Out, Cell Characterization, Flow Cytometry, Expressing, In Vitro, Isolation, Incubation